Introduction to NGS

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About the Product

What is Next-Generation Sequencing?

Next-Generation Sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. NGS has revolutionized the biological sciences, allowing labs to perform a wide variety of applications and study biological systems at a level never before possible. Today's complex genomics questions demand a depth of information beyond the capacity of traditional DNA sequencing technologies. NGS has filled that gap and become an everyday tool to address these questions.

Our Product

We Provide Various Directions

  • Overview
  • Applications
  • Documentation
  • Technology
  • Using NGS
  • Resources
Overview
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How does Illumina NGS work?

Illumina NGS technology utilizes a fundamentally different approach from the classic Sanger chain-termination method. It leverages sequencing by synthesis (SBS) technology – tracking the addition of labeled nucleotides as the DNA chain is copied – in a massively parallel fashion.

Next-generation sequencing generates masses of DNA sequencing data, and is both less expensive and less time-consuming than traditional Sanger sequencing.2 Illumina sequencing systems can deliver data output ranging from 300 kilobases up to multiple terabases in a single run, depending on instrument type and configuration.

In Depth NGS Instruction book. Click for More.

Applications
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Applications of NGS

Next-generation sequencing technology has fundamentally changed the kinds of questions scientists can ask and answer. Innovative sample preparation and data analysis options enable a broad range of applications. For example, NGS allows labs to:

  • Rapidly sequence whole genomes
  • Deeply sequence target regions
  • Utilize RNA sequencing (RNA-Seq) to discover novel RNA variants and splice sites, or quantify mRNAs for gene expression analysis
  • Analyze epigenetic factors such as genome-wide DNA methylation and DNA-protein interactions
  • Sequence cancer samples to study rare somatic variants, tumor subclones, and more
  • Study the human microbiome
  • Identify novel pathogens

 

Learn More about NGS Applications

Documentation
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NGS workflow

The next-generation sequencing workflow includes three basic steps: Library preparation, Sequencing, and Data Analysis.

Technology
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Advances in NGS technology

Recent Illumina next-generation sequencing technology breakthroughs include:

  • XLEAP-SBS chemistry: This innovation delivers increased speed and greater fidelity compared to standard Illumina sequencing by synthesis (SBS) chemistry.
  • Up to 16 Tb: The NovaSeq X Series provides extraordinary sequencing power to fuel data-intensive applications.  
  • Semiconductor sequencing: This technology combines a complementary metal-oxide semiconductor (CMOS) chip with one-channel SBS to deliver high-accuracy data in a compact instrument.
  • Patterned flow cell technology: This advance provides an exceptional level of throughput for diverse sequencing applications. 
Using NGS
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Instrument buying guidance

The resources below offer valuable guidance to scientists who are considering purchasing a next-generation sequencing instrument.

NGS experimental considerations

Learn about read length, coverage, quality scores, and other experimental considerations to help you plan your sequencing run.

Use our interactive tools to help you create a custom NGS protocol or select the right products and methods for your project.

Resources
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Resources for high-throughput NGS labs

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